Development and Validation of Analytical Methods for Alprazolam and Fluoxetine In Pharmaceutical Dosage Form

Alprazolam (ALP) is benzodiazepine derivative which produce symptomatic relief of anxiety and tension state resulting from a stressful enviourment or emotional factors. The First method was based on simultaneous equation method.  In the proposed simultaneous equation method, the signals were measured at 222.0 nm and 227.0 nm corresponding to the absorbance maxima of ALP and FLU in methanol.  The method was validated statistically.  Recovery study was performed to confirm the accuracy of the method.  This method is suitable technique for the reliable analysis of commercial formulation containing combination of ALP and FLU.  Simplicity, sensitivity and rapidity of simultaneous equation method render it suitable for routine analysis for ALP and FLU from their combination dosage forms. The Second method was based on absorbance ratio method (Q. analysis).  The absorbance was measured at two-wave lengths 222.0 nm (max) of ALP and 227.0 nm, which is an isoabsorptive point, were found adequate for quantification.  The method is validated statistically. The recovery more than 99% with low SD suggests the accuracy of the method. In addition, the above-proposed UV spectroscopic methods are simple, easy to apply, low cost, does not use polluting reagents and requires relatively inexpensive instruments.