Simultaneous determination of Ceftriaxone and Cefpodoximeproxetil in Commercial Formulations and Spiked Human Plasma using Reversed-Phase High Performance Liquid Chromatography

A simple and sensitive reversed phase high performance liquid chromatographic (RP-HPLC) method has been developed for the simultaneous quantification of ceftriaxone and cefpodoximeproxetil in artificial mixtures of commercial formulations and spiked human plasma. The separation was carried on C18 (4.6 mm x 250 mm) column with a mixture of acetonitrile and 0.03 M triethylamine (3:1 v/v) adjusted to pH 7.0, with acetic acid, as mobile phase at flow rate of 0.9 mLmin‒1 with UV detector at 240 nm. The chromatographic peaks were recorded at detection wavelength of 240 nm. The retention times of ceftriaxone and cefpodoximeproxetil were 2.111±0.014 min and 4.053±0.013 min respectively. The concentration versus detector response (Peak height) curve is linear in the range of 0.5- 250 µg mL‒1 for ceftriaxone (R2=0.9996), and 0.5-500 µg mL‒1 for cefpodoximeproxetil (R2=0.9995). The limit of detection (3.3σ/S) was found to be 6.62 ng mL‒1 and 14.2 ngmL‒1, respectively, for ceftriaxone and cefpodoximeproxetil. Similarly limit of quantification (10σ/S) was 20 ng mL‒1 for ceftriaxone and 42.9 ngmL‒1 for cefpodoximeproxetil. The developed method was applied to simultaneous determination of these antimicrobials in the artificial mixtures of commercial formulations and spiked human plasma.