Studies on the Control of Mosquito, Aedes Aegypti using the Ethanol Seed Extract of Annona Reticulata As A Biocide

In the present study the seeds of Annona reticulata was extracted with ethanol, and evaluated for mosquitocidal effect against the dengue vector Aedes aegypti. The preliminary phytochemical analysis showed the presence of alkaloids, flavanoids, glycosides, steroids and phenols in the ethanol seed extract of Annona reticulata. The percentage of egg hatchability in ethanol seed extract was 73% in 0.1%, 59% in 0.2% 42% in 0.3%, and 25% in 0.4%, 11% in 0.5% respectively and 100% hatchability was seen in control. The level of the toxicity of Annona reticulata was expressed in terms of LC50/24 hour value. The LC50 values of ethanol seed extract for I, III & IV instar larvae  and pupae of Aedes aegypti were 0.717 %, 1.263 %, 1.369 %, 1.425 % respectively. The morphogenetic abnormalities are commonly caused by botanical extracts and the disturbance from the growth regulating hormones. It is therefore suggested that Annona reticulata derivatives are considered for vector control operations besides their use in other fields after exploring field trails.